Phytosanitary Diagnosis
This service aims to support the entire agribusiness production chain in decision-making and control of phytosanitary problems, based on an accurate diagnosis.
We perform Phytosanitary Diagnosis on agricultural products and by-products from import, export, official survey, internal transit, quality control, clinical and laboratory diagnosis and seed pathology programs.
Laboratory analyses are based on biochemical, physical, biological, morphological and molecular methods (Conventional and real-time PCRs, LAMP, Sequencing) for the detection and identification of various pathosystems.
We diagnose plant diseases and identificaty of pests (fungi, oomycetes, prokaryotes, viruses, viroids, nematodes, insects, mites, seeds and plants of invasive species) in agricultural products and by-products, through clinical and laboratory analyses that meet the regulatory requirements related to national and international transit, as well as the phytosanitary quality control of agricultural products. Check the possibilities in the table below.
| Service | Purpose |
|---|---|
| Mites | Mites Mite detection by visual examination + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material |
| Mite detection by sieving + identification by PCR and sequencing | |
| Mite detection by visual examination + identification by PCR and sequencing | |
| Bacteria | Bacteria Detection of bacteria by pure culture + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material |
| Detection of bacteria by pure culture + identification by biochemical tests | |
| Detection of bacteria by pure culture + identification by PCR and sequencing | |
| Detection of bacteria by pure culture + identification by PCR and sequencing | |
| Detection + identification of bacteria by PCR | |
| Detection + identification of bacteria by qPCR | |
| Detection + identification of bacteria by PCR and sequencing | |
| Fungi/Oomycetes | Fungi/Oomycetes Detection of fungus/oomycete by observation of symptoms and signs + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material |
| Detection of fungus/oomycete by pure culture + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material | |
| Detection of fungus by Blotter Test + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material | |
| Detection of fungus by Blotter Test + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material | |
| Detection of oomycete by biological baits and identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material | |
| Detection of fungus/oomycete by pure culture + identification by PCR and sequencing | |
| Detection of fungus/oomycete by pure culture + identification by PCR and sequencing | |
| Detection + identification of fungus/oomycete by PCR and sequencing | |
| Insect | Insect Insect detection by visual examination + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material |
| Insect detection by visual examination + identification by PCR and sequencing | |
| Insect detection by visual examination + identification by qPCR | |
| Mollusks | Mollusks Detection by visual examination + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material |
| Nematodes | Nematodes Nematode detection by Coolen & D'Herde + identification by observation of morphology under microscopy and/or Consultation of bibliography and/or reference material |
| Nematode detection by Coolen & D'Herde + identification by PCR and sequencing | |
| Nematode detection by Coolen & D'Herde + identification by qPCR | |
| Detection of nematode by Jenkins + identification by observation of morphology under microscopy and/or Consultation of bibliography and/or reference material | |
| Jenkins nematode detection + PCR identification and sequencing | |
| Jenkins nematode detection + identification by qPCR | |
| Invasive Seeds | Invasive Seeds Detection of invasive seed by visual examination + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material |
| Detection of invasive seed by visual examination + identification by PCR and sequencing | |
| Detection of invasive seed by visual examination + identification by qPCR | |
| Viruses/Viroid | Viruses/Viroid Detection + identification of viruses by PCR and RCA |
| PCR virus detection + identification sequencing | |
| Detection + identification of virus/viroid by qPCR | |
| RT-PCR virus/viroid detection + identification and sequencing | |
| Virus/viroid detection + identification by RT-qPCR |
Import
Identification and characterization of pests in agricultural products and by-products related to the official programs of MAPA (Brazilian Ministry of Agriculture, Livestock and Food Supply) in compliance with import processes. We have the necessary expertise to identify more than 800 regulated pests for Brazil described in around 300 normative acts of import authorization.
Export
Identification and characterization of pests in agricultural products and by-products related to export processes. We have the necessary expertise to identify more than 700 types of restriction pests, provided for in bilateral agreements between countries.
Clinical laboratory diagnosis and seed pathology
Identification of possible pathogens associated with signs and symptoms in (parts of) plants, and/or seeds. This service may be requested when the client is unaware of the causal agent. In seed pathology analyses, we use the blotter test and/or molecular analysis via real-time PCR (qPCR) to assist in the detection and identification of pathogens.
Quality control
Service aimed at monitoring the quality standards defined by the applicant, from an analysis of pre-defined targets.
Official survey
Survey of pests with high potential for economic damage, officially controlled by Federal Superintendencies of Agriculture and State Agencies of Plant Health Defense. In recent years, the Agronomic Laboratory has been collaborating with official pest monitoring programs in at least 10 Brazilian states. There are more than 23 species of agricultural interest, being 11 species of fungi, 6 bacteria, 2 viruses, 2 invasive plants and 1 species of nematode.
| Group | Disease/Pathogen |
|---|---|
| Mite | Citrus Hindu mite (Schizotetranychus hindustanicus) |
| Insect | Carambola fruit fly (Bactrocera carambolae) |
| West Indian Cherry Weevil (Anthonomus tomentosus) | |
| Citrus blackfly (Aleurocanthus woglumi) | |
| Mango seed weevil (Sternochetus mangiferae) | |
| Fungi | Fungi Citrus Black Spot (Phyllosticta citricarpa (= Guinardia citricarpa)) |
| Black Sigatoka (Pseudocercospora fijiensis (= Mycosphaerella fijiensis)) | |
| Nectria canker (Neonectria ditissima (= Neonectria galligena)) | |
| Monilinia (Moniliophthora roreri) | |
| Banana fusariosis, or Panama disease (Fusarium oxysporum f. sp. cubense, tropical breed 4) | |
| Asian soybean rust (Phakopsora pachyrhizi) | |
| Bacteria | Bacteria Citrus huanglongbing, or greening disease (Candidatus Liberibacter americanus) |
| Citrus huanglongbing, or greening disease (Candidatus Liberibacter asiaticus) | |
| Moko disease (Ralstonia solanacearum breed 2) | |
| Citrus canker (Xanthomonas citri subsp. citri) | |
| Bacterial canker of the vine (Xanthomonas campestris pv. viticola) | |
| Weed | Amaranthus palmeri |
| Insect | Insect Insect detection by visual examination + identification by observation of morphology under microscopy and/or consultation of bibliography and/or reference material |
| Insect detection by visual examination + identification by PCR and sequencing | |
| Insect detection by visual examination + identification by qPCR | |
| Nematode | Nematode Root-knot nematodes (Meloidogyne spp.) |
| Virus | Virus Papaya honeydew (Papaya meleira virus) |
| Papaya mosaic (Papaya ring spot virus) |
Internal transit
Related to the process of evaluation and inspection of products that are being marketed or moved within national borders.
| Crop | Plague |
|---|---|
| Banana tree | Cucumber mosaic virus |
| Banana streak virus | |
| Potato | Potato Black scurf (Rhizoctonia solani = Thanatephorus cucumeris) |
| Common scab (Streptomyces spp.) | |
| Silver scurf (Helminthosporium solani) | |
| Soft rot, Black cinnamon, Hollow stem (Pectobacterium spp; Dickeya spp.) | |
| Bacterial wilt disease (Ralstonia solanacearum) | |
| Powdery scab (Spongospora subterranea) | |
| Late blight (Phytopthora infestans) | |
| Dry rot (Fusarium spp.) | |
| Black spot (Altenaria solani) | |
| Brown Eye (Cylindrocladium spp.) | |
| Root-knot nematodes (Meloidogyne spp.) | |
| Lesion nematodes (Pratylenchus spp.) | |
| Damage caused by other insects | |
| Aphids | |
| PVX | |
| PVY | |
| PVS | |
| PLRV |
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Company
Who We Are
Agronômica is a reference in phytosanitary diagnosis, acting in a complementary way to the official demands of the Ministry of Agriculture, Livestock and Supply – MAPA.
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